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organ culture medium (dmem/f12)  (Thermo Fisher)


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    Structured Review

    Thermo Fisher organ culture medium (dmem/f12)
    Organ Culture Medium (Dmem/F12), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/organ+culture+medium/dmem+f12/pmc12045629-221-31-36
    Average 90 stars, based on 1 article reviews
    organ culture medium (dmem/f12) - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: RET-mediated glial cell line-derived neurotrophic factor signaling inhibits mouse prostate development
    Article Snippet: .. The PCR was achieved in the presence of 1 M betaine using the following method: a denaturation and polymerase activation step at 94°C for 1 min and then 40 cycles consisting of 94°C for 10 s, 62°C for 10 s and 72°C for 20 s. Urogenital sinus (UGS) and prostate organ culture UGSs were dissected from E15.5 male embryos and prostates were collected from P0 neonates in DMEM/F12 (1:1) medium (21041-025, Invitrogen) and cultured on 0.4 μm Millicell CM filters (PICM01250, EMD Millipore) floating on organ culture medium [DMEM/F12 supplemented with 1% insulin, transferrin and selenium (51500-056, Invitrogen), and gentamicin and amphotericin B (R-015-10, Invitrogen)] in a 5% CO 2 atmosphere, as described previously ( Park and Bolton, 2015 ). .. Additional supplements included 0.1% ethanol control, 10 nM DHT (A8380, Sigma Aldrich) in ethanol, 0.1% PBS control and 1-100 ng/ml recombinant GDNF (212-GD, R&D Systems) in PBS.

    Activation Assay:

    Article Title: RET-mediated glial cell line-derived neurotrophic factor signaling inhibits mouse prostate development
    Article Snippet: .. The PCR was achieved in the presence of 1 M betaine using the following method: a denaturation and polymerase activation step at 94°C for 1 min and then 40 cycles consisting of 94°C for 10 s, 62°C for 10 s and 72°C for 20 s. Urogenital sinus (UGS) and prostate organ culture UGSs were dissected from E15.5 male embryos and prostates were collected from P0 neonates in DMEM/F12 (1:1) medium (21041-025, Invitrogen) and cultured on 0.4 μm Millicell CM filters (PICM01250, EMD Millipore) floating on organ culture medium [DMEM/F12 supplemented with 1% insulin, transferrin and selenium (51500-056, Invitrogen), and gentamicin and amphotericin B (R-015-10, Invitrogen)] in a 5% CO 2 atmosphere, as described previously ( Park and Bolton, 2015 ). .. Additional supplements included 0.1% ethanol control, 10 nM DHT (A8380, Sigma Aldrich) in ethanol, 0.1% PBS control and 1-100 ng/ml recombinant GDNF (212-GD, R&D Systems) in PBS.

    Organ Culture:

    Article Title: RET-mediated glial cell line-derived neurotrophic factor signaling inhibits mouse prostate development
    Article Snippet: .. The PCR was achieved in the presence of 1 M betaine using the following method: a denaturation and polymerase activation step at 94°C for 1 min and then 40 cycles consisting of 94°C for 10 s, 62°C for 10 s and 72°C for 20 s. Urogenital sinus (UGS) and prostate organ culture UGSs were dissected from E15.5 male embryos and prostates were collected from P0 neonates in DMEM/F12 (1:1) medium (21041-025, Invitrogen) and cultured on 0.4 μm Millicell CM filters (PICM01250, EMD Millipore) floating on organ culture medium [DMEM/F12 supplemented with 1% insulin, transferrin and selenium (51500-056, Invitrogen), and gentamicin and amphotericin B (R-015-10, Invitrogen)] in a 5% CO 2 atmosphere, as described previously ( Park and Bolton, 2015 ). .. Additional supplements included 0.1% ethanol control, 10 nM DHT (A8380, Sigma Aldrich) in ethanol, 0.1% PBS control and 1-100 ng/ml recombinant GDNF (212-GD, R&D Systems) in PBS.

    Article Title: Chlamydophila pneumoniae Infection Leads to Smooth Muscle Cell Proliferation and Thickening in the Coronary Artery without Contributions from a Host Immune Response
    Article Snippet: The left anterior descending coronary artery was flushed with standard phosphate saline buffer (PBS) supplemented with an antibiotic mixture (penicillin G ,1500 units/ml; streptomycin sulfate, 1500 μg/ml; amphotericin B, 2.5 μg/ml; Invitrogen) and subsequently dissected out of the heart and cleaned of adhering fat and connective tissue. .. The explanted artery was cut into segments of 5 mm in length and incubated at 37°C and 5% CO 2 in organ culture medium (Dulbecco’s Modified Eagle’s Medium; Invitrogen, supplemented with 20% fetal bovine serum; Hyclone) supplemented with antibiotics (penicillin G ,1050 units/ml; streptomycin sulfate, 1050 μg/ml; amphotericin B, 2.5 μg/ml; Invitrogen). ..

    Article Title: RET-mediated glial cell line-derived neurotrophic factor signaling inhibits mouse prostate development
    Article Snippet: .. UGSs were dissected from E15.5 male embryos and prostates were collected from P0 neonates in DMEM/F12 (1:1) medium (21041-025, Invitrogen) and cultured on 0.4 μm Millicell CM filters (PICM01250, EMD Millipore) floating on organ culture medium [DMEM/F12 supplemented with 1% insulin, transferrin and selenium (51500-056, Invitrogen), and gentamicin and amphotericin B (R-015-10, Invitrogen)] in a 5% CO 2 atmosphere, as described previously ( Park and Bolton, 2015 ). .. Additional supplements included 0.1% ethanol control, 10 nM DHT (A8380, Sigma Aldrich) in ethanol, 0.1% PBS control and 1-100 ng/ml recombinant GDNF (212-GD, R&D Systems) in PBS.

    Article Title: Glial Cell Line-Derived Neurotrophic Factor Induces Cell Proliferation in the Mouse Urogenital Sinus
    Article Snippet: .. UGS organ culture UGS tissue was dissected from E15.5 male embryos and placed on 0.4 μm Millicell CM filters (catalog number PICM01250; EMD Millipore), floating on 1 mL of organ culture medium [DMEM/F12 supplemented with 1% nonessential amino acids, 2% insulin, transferrin and selenium (catalog number 51500-056; Invitrogen), gentamicin and amphotericin B (catalog number R-015-10; Invitrogen), and 1 g/L of D-glucose] in a 5% CO 2 atmosphere ( 70 , 71 ). .. Additional supplements included 0.1% ethanol control, 10 nM 5α-dihydrotestosterone (DHT; catalog number A8380; Sigma-Aldrich) in ethanol, 0.1% PBS control, 1–100 ng/mL recombinant GDNF (catalog number 212-GD; R&D Systems) in PBS, 100 ng/mL recombinant neurturin (NRTN; catalog number 1297-NE; R&D Systems) in PBS, 0.1% dimethylsulfoxide (DMSO) control, 20 μM U0126 (catalog number 1144; Tocris Bioscience) in DMSO, 20 μM PD98059 (catalog number 1213; Tocris Bioscience) in DMSO, and 100 μM 1,3-dihydro-5,6-dimethoxy-3-[(4-hydrophenyl)methylene]1-H-indol-2-one (RPI-1; catalog number R8907; Sigma-Aldrich) in DMSO.

    Article Title: Glial Cell Line-Derived Neurotrophic Factor Induces Cell Proliferation in the Mouse Urogenital Sinus
    Article Snippet: .. UGS tissue was dissected from E15.5 male embryos and placed on 0.4 μm Millicell CM filters (catalog number PICM01250; EMD Millipore), floating on 1 mL of organ culture medium [DMEM/F12 supplemented with 1% nonessential amino acids, 2% insulin, transferrin and selenium (catalog number 51500-056; Invitrogen), gentamicin and amphotericin B (catalog number R-015-10; Invitrogen), and 1 g/L of D-glucose] in a 5% CO 2 atmosphere ( 70 , 71 ). .. Additional supplements included 0.1% ethanol control, 10 nM 5α-dihydrotestosterone (DHT; catalog number A8380; Sigma-Aldrich) in ethanol, 0.1% PBS control, 1–100 ng/mL recombinant GDNF (catalog number 212-GD; R&D Systems) in PBS, 100 ng/mL recombinant neurturin (NRTN; catalog number 1297-NE; R&D Systems) in PBS, 0.1% dimethylsulfoxide (DMSO) control, 20 μM U0126 (catalog number 1144; Tocris Bioscience) in DMSO, 20 μM PD98059 (catalog number 1213; Tocris Bioscience) in DMSO, and 100 μM 1,3-dihydro-5,6-dimethoxy-3-[(4-hydrophenyl)methylene]1-H-indol-2-one (RPI-1; catalog number R8907; Sigma-Aldrich) in DMSO.

    Cell Culture:

    Article Title: RET-mediated glial cell line-derived neurotrophic factor signaling inhibits mouse prostate development
    Article Snippet: .. The PCR was achieved in the presence of 1 M betaine using the following method: a denaturation and polymerase activation step at 94°C for 1 min and then 40 cycles consisting of 94°C for 10 s, 62°C for 10 s and 72°C for 20 s. Urogenital sinus (UGS) and prostate organ culture UGSs were dissected from E15.5 male embryos and prostates were collected from P0 neonates in DMEM/F12 (1:1) medium (21041-025, Invitrogen) and cultured on 0.4 μm Millicell CM filters (PICM01250, EMD Millipore) floating on organ culture medium [DMEM/F12 supplemented with 1% insulin, transferrin and selenium (51500-056, Invitrogen), and gentamicin and amphotericin B (R-015-10, Invitrogen)] in a 5% CO 2 atmosphere, as described previously ( Park and Bolton, 2015 ). .. Additional supplements included 0.1% ethanol control, 10 nM DHT (A8380, Sigma Aldrich) in ethanol, 0.1% PBS control and 1-100 ng/ml recombinant GDNF (212-GD, R&D Systems) in PBS.

    Article Title: RET-mediated glial cell line-derived neurotrophic factor signaling inhibits mouse prostate development
    Article Snippet: .. UGSs were dissected from E15.5 male embryos and prostates were collected from P0 neonates in DMEM/F12 (1:1) medium (21041-025, Invitrogen) and cultured on 0.4 μm Millicell CM filters (PICM01250, EMD Millipore) floating on organ culture medium [DMEM/F12 supplemented with 1% insulin, transferrin and selenium (51500-056, Invitrogen), and gentamicin and amphotericin B (R-015-10, Invitrogen)] in a 5% CO 2 atmosphere, as described previously ( Park and Bolton, 2015 ). .. Additional supplements included 0.1% ethanol control, 10 nM DHT (A8380, Sigma Aldrich) in ethanol, 0.1% PBS control and 1-100 ng/ml recombinant GDNF (212-GD, R&D Systems) in PBS.

    Incubation:

    Article Title: Chlamydophila pneumoniae Infection Leads to Smooth Muscle Cell Proliferation and Thickening in the Coronary Artery without Contributions from a Host Immune Response
    Article Snippet: The left anterior descending coronary artery was flushed with standard phosphate saline buffer (PBS) supplemented with an antibiotic mixture (penicillin G ,1500 units/ml; streptomycin sulfate, 1500 μg/ml; amphotericin B, 2.5 μg/ml; Invitrogen) and subsequently dissected out of the heart and cleaned of adhering fat and connective tissue. .. The explanted artery was cut into segments of 5 mm in length and incubated at 37°C and 5% CO 2 in organ culture medium (Dulbecco’s Modified Eagle’s Medium; Invitrogen, supplemented with 20% fetal bovine serum; Hyclone) supplemented with antibiotics (penicillin G ,1050 units/ml; streptomycin sulfate, 1050 μg/ml; amphotericin B, 2.5 μg/ml; Invitrogen). ..

    Modification:

    Article Title: Chlamydophila pneumoniae Infection Leads to Smooth Muscle Cell Proliferation and Thickening in the Coronary Artery without Contributions from a Host Immune Response
    Article Snippet: The left anterior descending coronary artery was flushed with standard phosphate saline buffer (PBS) supplemented with an antibiotic mixture (penicillin G ,1500 units/ml; streptomycin sulfate, 1500 μg/ml; amphotericin B, 2.5 μg/ml; Invitrogen) and subsequently dissected out of the heart and cleaned of adhering fat and connective tissue. .. The explanted artery was cut into segments of 5 mm in length and incubated at 37°C and 5% CO 2 in organ culture medium (Dulbecco’s Modified Eagle’s Medium; Invitrogen, supplemented with 20% fetal bovine serum; Hyclone) supplemented with antibiotics (penicillin G ,1050 units/ml; streptomycin sulfate, 1050 μg/ml; amphotericin B, 2.5 μg/ml; Invitrogen). ..



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